Journal: Animals : an Open Access Journal from MDPI

Article Title: A Functional 3′ UTR Polymorphism of FADS2 Affects Cow Milk Composition through Modifying Mir-744 Binding

doi: 10.3390/ani9121090

Figure Lengend Snippet: SNP c.1571G>A affects the binding of miR-744 to FADS2 . ( A ) Schematic representation of the dual luciferase constructs harboring different alleles. ( B , C ) Relative luciferase activities are decreased in the presence of the G allele and miR-744, as compared with the A allele and miR-744, in MAC-T ( B ) and HEK293T cells ( C ). Error bars (SE) are derived from three independent experiments performed in triplicate, and data were compared using a Student’s t -test. * p < 0.05, ** p < 0.01.

Article Snippet: The original bovine mammary epithelial cells (MAC-T) were purchased at Nexia Biotechnologies (Quebec, QC, Canada).

Techniques: Binding Assay, Luciferase, Construct, Derivative Assay

Journal: Animals : an Open Access Journal from MDPI

Article Title: A Functional 3′ UTR Polymorphism of FADS2 Affects Cow Milk Composition through Modifying Mir-744 Binding

doi: 10.3390/ani9121090

Figure Lengend Snippet: Influence of miR-744 on endogenous FADS2 expression in MAC-T cells. ( A – D ) MAC-T cells were transfected with a control miRNA, miR-744 mimic, or miR-744 inhibitor as indicated. Twenty-four hours after transfection, the relative FADS2 mRNA level was measured by RT-qPCR ( A , C ). Error bars (SE) are derived from three independent experiments performed in triplicate, and data were compared using a Student’s t -test. * p < 0.05, *** p < 0.001. Forty-eight hours after transfection, FADS2 protein expression was examined by Western blot ( B , D ).

Article Snippet: The original bovine mammary epithelial cells (MAC-T) were purchased at Nexia Biotechnologies (Quebec, QC, Canada).

Techniques: Expressing, Transfection, Quantitative RT-PCR, Derivative Assay, Western Blot